Dopamine, an organic chemical belonging to the catecholamine-phenethylamine family, plays a variety of important roles in the body and brain. Dopamine is a neurotransmitter in the brain. Dopamine is a local chemical messenger that functions outside the central nervous system. It is made by your body, and the nervous system.
Dopamine ELISA Kit is a competitive ELISA test that measures Dopamine levels in plasma, serum and cell culture supernatants. Dopamine ELISA kits are assays designed to detect the neurotransmitter and hormone dopamine, also known as 3,4-dihydroxyphenethylamine.
It is most closely associated with the brain, its pathways, and plays a role in conditions such as Parkinson's disease, schizophrenia, attention deficit hyperactivity disorder (ADHD), and other mental disorders. It can be used to treat shock and heart failure.
The Dopamine ELISA Kit is a colorimetric immunoassay tool that provides results within 2 hours. It uses the microtiter plates format. Dopamine is bound with the microtiter plates solid phase. The solid phase bound dopamine and the sample dopamine are competing for a limited number of antiserum-binding sites.
Washing is used to remove any antigen or antigen-antiserum compounds that are not in equilibrium. Anti-rabbit IgG/peroxidase detects the antibody bound to dopamine's solid phase. At 450 nm, the substrate TMB/peroxidase reaction can be observed. The dopamine concentration in the sample is directly proportional to how much antibody is bound to its solid phase.